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Molecular Analysis of Cancer Jacqueline Boultwood

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Livre - Jacqueline Boultwood - 01/10/2001 - Langue : Anglais

Résumé : Leading researchers describe in step-by-step detail their best state-of-the art molecular techniques for elucidating the biochemical processes associated with human cancer. Among...

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    • Auteur(s) : Jacqueline Boultwood - Carrie Fidler
    • Editeur : Humana Press
    • Langue : Anglais
    • Parution : 01/10/2001
    • Nombre de pages : 316
    • Expédition : 664
    • Dimensions : 22.9 x 15.2 x 2.5

    Résumé :
    Leading researchers describe in step-by-step detail their best state-of-the art molecular techniques for elucidating the biochemical processes associated with human cancer. Among the techniques presented for identifying chromosomal rearrangements are fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), microsatellite analysis, PCR and RT-PCR, and DNA fingerprinting. Additional methods include SSCP, DGGE, the nonisotopic RNase cleavage assay, and the protein truncation assay and DNA sequencing for identifying mutations.

    Molecular Analysis of Cancer: An Overview Ken Mills Detection of Chromosome Abnormalities in Leukemia Using Fluorescence In Situ Hybridization Lyndal Kearney, Sabrina Tosi, and Rina J. Jaju Spectral Karyotyping in Cancer Cytogenetics Eva Hilgenfeld, Cristina Montagna, Hesed Padilla-Nash, Linda Stapleton, Kerstin Heselmeyer-Haddad, and Thomas Ried Comparative Genomic Hybridization Analysis Binaifer R. Balsara, Jianming Pei, and Joseph R. Testa Detection of Chromosomal Deletions by Microsatellite Analysis Rachel E. Ibbotson and Martin M. Corcoran Detection and Quantification of Leukemia-Specific Rearrangements Andreas Hochhaus Detection of t(2;5)(p23;q35) Translocation by Long-Range PCR of Genomic DNA Yunfang Jiang, L. Jeffrey Medeiros, and Andreas H. Sarris Use of DNA Fingerprinting to Detect Genetic Rearrangements in Human Cancer Vorapan Sirivatanauksorn, Yongyut Sirivatanauksorn, Arthur B. McKie, and Nicholas R. Lemoine Mutation Analysis of Large Genomic Regions in Tumor DNA Using Single-Strand Conformation Polymorphism: Lessons from the ATM Gene Igor Vorechovsky Mutational Analysis of Oncogenes and Tumor Suppressor Genes in Human Cancer Using Denaturing Gradient Gel Electrophoresis Per Guldberg, Kirsten Gr?nb?k, Jesper Worm, Per thor Straten, and Jesper Zeuthen Detection of Mutations in Human Cancer Using Nonisotopic RNase Cleavage Assay Marianna Goldrick and James Prescott Mutational Analysis of the Neurofibromatosis Type 1 Gene in Childhood Myelodysplastic Syndromes Using a Protein Truncation Assay Lucy Side Mutation Analysis of Cancer Using Automated Sequencing Amanda Strickson and Carrie Fidler Detection of Differentially Expressed Genes in Cancer Using Differential Display Yineng Fu Genomewide Gene Expression Analysis Using cDNAMicroarrays Chuang Fong Kong and David Bowtell Gene Expression Profiling in Cancer Using cDNA Microarrays Javed Khan, Lao H. Saal, Michael L. Bittner, Yuan Jiang, Gerald C. Gooden, Arthur A. Glatfelter, and Paul S. Meltzer Wilms Tumor Gene WT1 as a Tumor Marker for Leukemic Blast Cells and Its Role in Leukemogenesis Haruo Sugiyama Detection of Aberrant Methylation of the p15INK4B Gene Promoter Toshiki Uchida Clonality Studies in Cancer Based on X Chromosome Inactivation Phenomenon John T. Phelan II and Josef T. Prchal Telomere Length Changes in Human Cancer Dominique Broccoli and Andrew K. Godwin Measurement of Telomerase Activity in Human Hematopoietic Cells and Neoplastic Disorders Kazuma Ohyashiki and Junko H. Ohyashiki

    The book covers a broad range of topics and provides many useful suggestions as well as practical guidance for the lab researcher. -Doody's Health Sciences Book Review Journal This book gives a good overview of currently available methods for investigating molecular changes in cancers, illustrates how you, the cancer researcher, can apply the methods to your oncology problem...The most enjoyable chapters also illustrate the methods with real results, so that you can see what to expect, e.g. chapter 3 on spectral karyotyping...a useful addition in any oncology research laboratory, because it presents this large variety of methods in a compact format, so that someone waiting to try a new approach could easily dip into the book to see what is available. - Molecular Biotechnology Comprehensive and state-of-the-art, Molecular Analysis of Cancer not only offers today's researcher a deeper understanding of cancer's molecular pathogenesis, but also provides a detailed examination of each of the major methodologies needed to detect and analyze its associated genetic abnormalities. - Clinical Laboratory ...good integration between preliminary explanations of techniques and practical instructions with easily available tables or figures. - Proteomics